Recombinant Expressed Vector pET32a (+) S Constructed by Ligation Independent Cloning
نویسندگان
چکیده
منابع مشابه
Recombinant expressed vector pET32a (+) S constructed by ligation independent cloning.
The aim of this work was to develop a new method for constructing vectors, named ligation-independent cloning (LIC) method. We constructed the S label expression vector and recombinant pET32a (+) S-phoN2 by LIC. The recombinant proteins were expressed in E. coli at a high level, and then the specificity of the recombinant proteins was identified by western blot. The target band was detected by ...
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ژورنال
عنوان ژورنال: Molecules
سال: 2014
ISSN: 1420-3049
DOI: 10.3390/molecules191016179